Kelch-like ECH-associated protein 1 (Keap1) is a substrate adapter protein in the Kelch-like (KLHL) family of proteins.{55226} It contains an N-terminal region, a BTB/POZ domain that facilitates protein binding and Keap1 dimerization, a central intervening region (IVR), a double glycine repeat (DGR)/Kelch repeat domain, a BACK that binds to other proteins, and a C-terminal region.{55226,53741,53742} KEAP1 is ubiquitously expressed and localized to the perinuclear region of the cytosol and bound to the actin skeleton via its DGR region.{53743,53744,53745} Under homeostatic conditions, it associates with Nrf2, preventing its nuclear translocation and promoting its ubiquitination and proteasomal degradation.{53741,53745} In the presence of electrophiles or oxidants, Keap1 releases Nrf2, which translocates to the nucleus to induce the expression of cytoprotective genes.{53745} Somatic mutations in Keap1 have been found in various cancers and human cancer cell lines and are associated with loss of Keap1 function and constitutive activation of Nrf2, which contributes to tumor growth and chemoresistance.{53742} Cayman’s Keap1 (human, recombinant) protein is comprised of Keap1 (amino acids 2-264) fused to His and GST tags at its N-terminus, consists of 860 amino acids, and has a calculated molecular weight of 97.37 kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass of this protein is approximately 109 kDa.
50 ug
Cayman Chemical
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