15-deoxy-d12,14-Prostaglandin J2-d4

15-deoxy-d12,14-PGJ2-d4 contains four deuterium atoms at the 3, 3', 4, and 4' positions. It is intended for use as an internal standard for the quantification of 15-deoxy-d12,14-PGJ2 by GC- or LC-mass spectrometry. 15-deoxy-d12,14-Prostaglandin J2 (15-deoxy-d12,14-PGJ2) is a metabolite of PGJ2. 15-deoxy-d12,14-PGJ2 is formed from PGD2 by the elimination of two molecules of water. It binds selectively to PPARy with an EC50 of 2 µM in a murine chimera system.{1424,1428} 15-deoxy-d12,14-PGJ2 is more potent than PGD2, d12-PGJ2, and PGJ2 in stimulating lipogenesis in C3H10T1/2 cells. The EC50 for induction of adipocyte differentiation in cultured fibroblasts is 7 µM.{1424}