Manidipine-d4

Manidipine-d4 is intended for use as an internal standard for the quantification of manidipine (Item No. 23614) by GC- or LC-MS. Manidipine is a dihydropyridine L- and T-type calcium channel blocker.{39642,39643,39644} It inhibits recombinant rabbit L-type (a1Ca2/dB1a) and human T-type (a1H) calcium channels expressed in Xenopus oocytes and native L-type channels in dissociated guinea pig cardiac ventricular cells (IC50 = 2.6 nM). Manidipine inhibits intracellular calcium increases induced by endothelin-1 (ET-1; Item No. 24127) in A7r5 rat vascular smooth muscle cells (ED50 = 1 nM) and potassium-induced contraction of isolated dog femoral and portal veins (IC50s = 24 and 2.1 nM, respectively).{39645,39646} In vivo, it lowers blood pressure in spontaneously hypertensive rats (SHRs) when administered at a dose of 10 mg/kg and inhibits left ventricular hypertrophy in rats induced by isoproterenol (Item No. 15592) when administered at a dose of 3 mg/kg.{39647,39648} Formulations containing manidipine have been used in the treatment of hypertension.