Activated neutrophils produce neutrophil extracellular traps (NETs), antimicrobial snares that are comprised of granular and cytoplasmic proteins, as well as decondensed nuclear DNA associated with histone proteins.{53539} NET degradation is impaired in patients with systemic lupus erythematosus (SLE), allowing for production of autoantibodies against NET components, including dsDNA. The NZBWF1 mouse model of SLE similarly produces autoantibodies against chromatin, dsDNA, and ssDNA.{53540,53541} Cayman's DNA Monoclonal Antibody (Clone 4E9) was developed by fusing the spleen of a non-immunized NZBWF1 mouse with a mouse myeloma cell line and can be used for immunohistochemistry (IHC), immunofluorescence (IF), and ELISA applications. The antibody recognizes ssDNA and dsDNA, including nuclear DNA, from human and bovine samples.
Specifications
Applications
IHC, ELISA, IF
Size
100 ug
Clone
4,00E+09
Host
Mouse
Supplier
Cayman Chemical
Shipping & storage
Shipping condition
Dry Ice
Storage temperature
-20°C