8-Hydroxypyrene-1,3,6-trisulfonic acid (HPTS) is a cell-impermeable fluorescent pH probe.{52382,22782} It displays excitation maxima of 403 and 450 nm, the intensities of which decrease and increase, respectively, as pH increases from 5 to 9 and an emission maximum of 510 nm.{52382} HPTS has been used in excitation ratio imaging to monitor changes in and measure the pH of endocytosed liposomes, acidic organelles, or the cytoplasm in live cell applications.{52382,22782} It is sensitive to fluorescence quenching by viologens, which can be reversed in the presence of glucose or other monosaccharides, and has been used as a glucose and carbohydrate sensor.{37199}
Specifications
Size
5 g
CAS number
6358-69-6
Supplier
Cayman Chemical
Shipping & storage
Shipping condition
Dry Ice
Storage temperature
-20°C