8-iso Prostaglandin F2a MaxSpec Standard

8-iso Prostaglandin F2a (8-iso PGF2a) is an isoprostane produced by the non-enzymatic peroxidation of arachidonic acid in membrane phospholipids.{320,371,855} It is present in human plasma in two distinct forms - esterified in phospholipids and as the free acid. The ratio of these two forms is approximately 2:1, with a total plasma 8-iso PGF2a level of about 150 pg/ml in normal volunteers. In normal human urine, 8-iso PGF2a levels are about 180-200 pg/mg of creatinine.{320,371} 8-iso PGF2a is a weak TP receptor agonist in vascular smooth muscle.{6640} Conversely, 8-iso PGF2a inhibits platelet aggregation induced by U-46619 (10-6 M) and I-BOP (3 x 10-7 M) with IC50 values of 1.6 x 10-6 M and 1.8 x 10-6 M, respectively.{855} 8-iso Prostaglandin F2a MaxSpec standard is a quantitative grade standard of 8-iso Prostaglandin F2a (Item No. 16350) that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This 8-iso Prostaglandin F2a MaxSpec standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.