4-Methylumbelliferyl-B-D-Glucopyranoside

4-Methylumbelliferyl-B-D-Glucopyranoside (4-MUG) is a fluorogenic substrate of B-glucosidase and B-glucocerebrosidase (also known as glucosylceramidase). In addition to its use in characterizing novel B-glucosidases, 4-MUG is used in assays to evaluate deficiency in B- glucocerebrosidase activity related to Gaucher disease.{32719,32720,32721} Hydrolysis of 4-MUG releases the fluorescent product 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.{27251}