5-(N-ethyl-N-isopropyl)-Amiloride

Sodium-hydrogen exchangers (NHE) are involved in maintaining sodium and pH balance in a variety of tissues. They are also known as sodium-hydrogen antiporters and solute carrier family 9 members. 5-(N-ethyl-N-isopropyl)-Amiloride (EIPA) is a potent inhibitor of several NHE isoforms, inhibiting NHE1, NHE2, NHE3, and NHE5 with Ki values of 0.02, 0.5, 2.4, and 0.42 ?M, respectively.{22706,22708} It less effectively inhibits NHE4 (IC50 ?10 ?M).{22706} EIPA is commonly used at a concentration of 5-10 ?M to inhibit cellular HNE activity.{22705,22707}