1-Stearoyl-2-Arachidonoyl-d8-sn-Glycerol

1-Stearoyl-2-arachidonoyl-d8-sn-glycerol (SAG-d8) is intended for use as an internal standard for the quantification of SAG (Item No. 10008650) by GC- or LC-MS. Many protein kinase C (PKC) isoforms require activation via second messengers including Ca2+, diacylglycerol (DAG), and/or a phospholipid in order to phosphorylate target proteins, initiating a variety of important signaling cascades.{943} 1-Stearoyl-2-arachidonoyl-sn-glycerol (SAG) is a DAG that contains the w-6 polyunsaturated fatty acid arachidonic acid in the sn-2 position and stearic acid in the sn-1 position of the glycerol backbone. It can potently activate PKCa, ?, and d at nM concentrations.{15530} Independent of PKC signaling, SAG competitively binds to the Ras activator RasGRP with a Ki value of 4.49 µM in Jurkat T cells.{15529}