One Lambda, NGS featuring AllType™ & NXType

AllType™ and NXType NGS assays offer a complete NGS solution that delivers unambiguous, high-resolution HLA genotyping results in less than three days.

NXType NGS kits

AllType™ NGS Assays 

  • NGS Based Genotyping with Fast Turnaround

AllType™ offers a complete NGS solution that delivers unambiguous, high-resolution genotyping results in less than three days. This assay delivers single tube amplification for all eleven Class I and Class II loci, completely removing the need for amplicon pooling. Samples are transferred directly to library preparation and made ready for sequencing in a single workday.

  • AllType™ NGS Assay on the Ion S5™ and  Illumina MiSeq™ Systems

The AllType workflow offers platform-specific barcoding to allow for added flexibility and sequencing on your system of choice. The speed and automation of the Ion Chef and S5 sequencing system delivers efficiency and reliability while TypeStream™ and TypeStream™ Visual, our two state-of-the-art data analysis softwares, automate genotyping to further shorten time-to-results.

FEATURES & BENEFITS:

  • High resolution genotyping of A, B, C, DRB1, DRB345, DQB1, DQA1, DPB1, and DPA1
  • No amplicon pooling — Direct to library preparation
  • One PCR reaction per sample
  • Compatible with mulitiple platforms
  • Up to 48 samples per sequencing run

AllType Workflow

AllType™ NGS Workflow

Watch the AllType™ video here

NXType Assays on Ion S5

NXType NGS HLA Typing Tests are optimized for use with the Ion Torrent™ Sequencing Technology to enable first pass, high-resolution genotyping of the HLA Class I and Class II loci. A Complete NGS Solution for Rapid Results.

FEATURES & BENEFITS:

  • Long range amplification (3.5 hr)
  • Requires only two reactions per sample for HLA A, B, C, DRB1, DRB3/4/5, DQB1, and DPB1 Loci
  • Up to 96 samples per sequencing run
  • Workflow suitable for low and high volume laboratories

NXType workflow

NXType NGS workflow

Watch the workflow video here

Benefits of the One Lambda NGS AllType™ and NXType workflows on the S5 sequencing platform:

  • Speed: Report genotyping results in less than 3 days
  • Simplicity: Shorten instrument setup time to less than 5 minutes
  • Scalability: Process up to 96 samples in a single run
  • Software: Shorten time to results with automated genotyping
  • Support: Get front line support for the entire workflow
One Lambda NXType NGS S5 Iontorrent   IonCHef
 The Ion S5 sequencing platform  The Ion Chef system

As the leader in HLA for over 30 years, One Lambda provides comprehensive hands-on user training and unmatched customer support. One Lambda NGS Workshops introduce researchers to next-generation sequencing technology and allow users to practice the entire workflow. Our customers are our number one priority and we’re proud to support One Lambda's complete NGS workflow from reagents to instruments and software.

We're here for you every step of the way.

Eager to learn more? Contact us or visit the One Lambda website for more information on the One Lambda NGS workflow featuring AllType™ and NXType reagents. 

 

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