Speed-oligo® allows for a visual and direct analysis, through a simple technique which provides results in just a few minutes. This PCR-based method coupled to a dipstick device enables a rapid, highly sensitive and specific detection.
This new technique, developed by Vircell, takes place in 3 steps: DNA extraction, amplification of the specific oligo pair (PCR) and detection of the amplified product with the strip.
Speed-oligo® contains all the reagents necessary to perform the PCR reaction and the detection step.
Principle of the test
The denatured PCR products (specific microorganism amplicon and control amplicon) are diluted in the hybridization solution and come into contact with the strip.
When flowing along the strip, the specific and control amplicons react with the complementary probes coupled to colloidal gold.
When reaching the visualization zone, the complexes of specific and control amplicons and the colloidal gold conjugates, react with a second probe which is bound to the membrane, leading to a coloured band. The excess of conjugate reacts with a complementary probe on the control line.
